| dc.creator | Anastassiadis,Savas | |
| dc.creator | Rehm,Hans-Jürgen | |
| dc.date | 2006-07-01 | |
| dc.date.accessioned | 2019-05-03T12:44:20Z | |
| dc.date.available | 2019-05-03T12:44:20Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/84902 | |
| dc.description | The influence of air saturation and temperature on continuous citric acid secretion was studied in chemostat cultures of Candida oleophila ATCC 20177 (var.). Simultaneous measurements of intra- and extracellular concentration of glucose, citric and isocitric acid confirmed the involvement of a specific active transport system in citrate secretion, favouring citric acid over isocitrate. An optimum air oxygen saturation of 20% and temperature of 30-31ºC were determined for the continuous citric acid secretion. The highest values of citric acid concentration (98 g/L), citrate to isocitrate ratio (33.3:1), volumetric citric productivity (1.8 g/(L x h)), and specific citric acid productivity (0.1 g/(g x h)), were reached at 20% air saturation at a residence time of 54 hrs by the experiment's lowest biomass of 18 g/L. The highest isocitic acid volumetric productivity (55.6 mg/(L x h)) and specific productivity (0.99 mg/(g x h)) were identified at 50%, instead. The fastest citrate excretion rate of the generic product of 0.046 g/(g*h) was found at 30-31ºC. A concentration ratio between extra- and intracellular concentration of citrate of up to 9 was identified. The highest extra-/intracellular ratio of citrate and lowest intracellular concentrations of glucose, citric and isocitric acid were determined at optimum air saturation as a consequence of active citrate export. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582006000400010 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.9 n.4 2006 | |
| dc.subject | Candida oleophila | |
| dc.subject | citric acid | |
| dc.subject | citric acid secretion | |
| dc.title | Oxygen and temperature effect on continuous citric acid secretion in Candida oleophila | |
