Designing and validation of genus-specific primers for human gut flora study

dc.creator	Rekha,Rani
dc.creator	Alam Rizvi,Moshahid
dc.creator	Jaishree,Paul
dc.date	2006-10-01
dc.date.accessioned	2019-05-03T12:44:20Z
dc.date.available	2019-05-03T12:44:20Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/84910
dc.description	The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
dc.format	text/html
dc.language	en
dc.publisher	Pontificia Universidad Católica de Valparaíso
dc.relation	10.4067/S0717-34582006000500005
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Electronic Journal of Biotechnology v.9 n.5 2006
dc.subject	16S rRNA based primers
dc.subject	anaerobic bacteria
dc.subject	diarrhoea patient
dc.subject	polymerase chain reaction
dc.title	Designing and validation of genus-specific primers for human gut flora study