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dc.creatorLungmann,Patcharee
dc.creatorChoorit,Wanna
dc.creatorPrasertsan,Poonsuk
dc.date2007-01-01
dc.date.accessioned2019-05-03T12:44:23Z
dc.date.available2019-05-03T12:44:23Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100001
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84924
dc.descriptionThe study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo components design containing 0.15-0.25 glucose, 0.15-0.25 yeast extract, 0.35-0.45 MgSO4·7H2O, 0.1-0.2 vitamin casamino acid and 0.02-0.06 KCl. The results of the mixture design revealed that the effect of nutrients or elements on the exopolymer produced by the strain SM5 were in the order of MgSO4·7H2O > yeast extract > vitamin casamino acid > KCl > glucose, respectively. The suitable medium recipe for enhancement the exopolymer production was 7.43 g/l glucose, 12.38 g/l yeast extract, 17.33 g/l MgSO4·7H2O, 9.9 g/l vitamin casamino acid and 2.48 g/l KCl. The exopolymer to be produced by the strain SM5 was 2.25 g/l, which was higher than that obtained in the original medium (1.3 times). The yield of exopolymer was 2.13 g/l to be obtained in medium containing 7.43 g/l glucose, 11.37 g/l yeast extract, 22.28 g/l MgSO4·7H2O, 7.44 g/l vitamin casamino acid and 0.99 g/l KCl which was predicted by response surface methodology. However, under an experiment, the yield of exopolymer was 2.08 ± 0.0020 g/l
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582007000100001
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.10 n.1 2007
dc.subjectexopolymer
dc.subjecthalophilic bacteria
dc.subjectmedium optimization
dc.subjectmixture design
dc.subjectresponse surface method
dc.titleApplication of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5


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