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dc.creatorVillena,Gretty K
dc.creatorGutiérrez-Correa,Marcel
dc.date2007-01-01
dc.date.accessioned2019-05-03T12:44:23Z
dc.date.available2019-05-03T12:44:23Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100012
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84935
dc.descriptionLignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2 IU mg-1 biomass) was observed in the presence of 20% ethylene glycol, suggesting differential regulatory mechanisms in biofilm fermentation related to cell adhesion.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582007000100012
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.10 n.1 2007
dc.subjectbiofilm
dc.subjectcellulase
dc.subjectethylene glycol
dc.subjectwater activity
dc.subjectxylanase
dc.titleProduction of lignocellulolytic enzymes by Aspergillus niger biofilms at variable water activities


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