| dc.creator | Sharma,Arun Dev | |
| dc.creator | Singh,Jaspreet | |
| dc.creator | Gill,Prabhjot Kaur | |
| dc.date | 2007-01-01 | |
| dc.date.accessioned | 2019-05-03T12:44:24Z | |
| dc.date.available | 2019-05-03T12:44:24Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/84939 | |
| dc.description | In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582007000100016 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.10 n.1 2007 | |
| dc.subject | bacteria | |
| dc.subject | ethanol | |
| dc.subject | transformation | |
| dc.title | Ethanol mediated enhancement in bacterial transformation | |
