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dc.creatorSwiech,Kamilla
dc.creatorda Silva,Gracinda M.C
dc.creatorZangirolami,Teresa C
dc.creatorIemma,Mônica R.C
dc.creatorSelistre-de-Araújo,Heloísa S
dc.creatorSuazo,Cláudio A.T
dc.date2007-04-01
dc.date.accessioned2019-05-03T12:44:25Z
dc.date.available2019-05-03T12:44:25Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200004
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84943
dc.descriptionWe present kinetic and physiological data regarding the culturing of rCHO-K1 cells on various microcarriers, to evaluate the potential of this culture strategy for mass production of these cells and expression of a recombinant disintegrin. Cultures were performed in 500 mL spinner flasks in DMEM culture medium with 10% v/v fetal calf serum, gently shaken at 37ºC, pH 7.4, in a 10% v/v CO2 atmosphere. The following values were obtained, respectively, for the adhesion time-constant Ka (h) and specific growth rate μmax (d-1) on each microcarrier: Cytodex 1 (0.91, 0.45), Cultispher S (0.28, 0.34), Immobasil FS (0.85, 0.52) and Pronectin F (5.12, 0.67). Metabolic characteristics showed some variation among the cultures with the four microcarriers, the most significant being the higher production of ammonia with microcarriers coated with adhesive molecules (Cultispher S and Pronectin F) relative to the uncoated carriers (Cytodex 1 and Immobasil FS). Experiments where the DMEM medium was gradually replaced by the serum-free medium (CHO-SFM-II) revealed important advantages over media containing serum, not only for assay purposes, but also for purification of the disintegrin. Altogether these results demonstrate that cultures on microcarriers, especially on Pronectin F, show good potential for larger scale cultures of rCHO-K1 cell
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582007000200004
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.10 n.2 2007
dc.subjectadhesion
dc.subjectanimal cell
dc.subjectCHO-K1
dc.subjectgrowth
dc.subjectmicrocarrier
dc.subjectrecombinant disintegrin
dc.titleEvaluating kinetic and physiological features of rCHO-K1 cells cultured on microcarriers for production of a recombinant metalloprotease/disintegrin


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