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  • Electronic Journal of Biotechnology
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Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922

Author
Kaur,Jatinder

Chadha,Bhupinder S

Kumar,Badhan A

Kaur,Ghatora, S

Saini,Harvinder S

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200010
Abstract
This study reports the purification and characterization of ß-glucosidase from a newly isolated thermophilic fungus, Melanocarpus sp. Microbial Type Culture Collection (MTCC) 3922. The molecular weight of ß-glucosidase was determined to be ~ 92 and 102 kDa with SDS PAGE and gel filtration, respectively, and pI of ~ 4.1. It was optimally active at 60ºC and pH 6.0, though was stable at 50ºC and pH 5.0 - 6.0. The presence of DTT, mercaptoethanol and metal ions such as Na+, K+, Ca2+, Mg2+and Zn2+ positively influenced the activity of ß-glucosidase but the activity was inhibited in the presence of CuSO4. ß-Glucosidase recognized pNP- ß-glucopyranoside (pNPG) as the preferred substrate, and showed very low affinity for pNP- ß-D-cellobioside. Km and Vmax for the hydrolysis of pNPG by ß-glucosidase was calculated as 3.3 mM and 43.68 µmolmin-1mg protein-1, respectively and k cat was quantified as 4 x 10³ min-1. ß-Glucosidase activity was enhanced appreciably in the presence of alcohols (methanol and ethanol) moreover, purified ß-glucosidase showed putative transglycosylation activity that was positively catalyzed in presence of methanol as an acceptor molecule
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Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
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Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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