Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter

dc.creator	Lavigne,Rob
dc.creator	Volckaert,Guido
dc.date	2007-04-01
dc.date.accessioned	2019-05-03T12:44:25Z
dc.date.available	2019-05-03T12:44:25Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200014
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/84953
dc.description	A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression
dc.format	text/html
dc.language	en
dc.publisher	Pontificia Universidad Católica de Valparaíso
dc.relation	10.4067/S0717-34582007000200014
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Electronic Journal of Biotechnology v.10 n.2 2007
dc.subject	cognate gene plasmid,
dc.subject	functional complementation
dc.subject	homolog
dc.subject	Saccharomyces cerevisiae
dc.subject	yeast
dc.title	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter