| dc.creator | Rasmussen-Poblete,Susana | |
| dc.creator | Valdes,Jorge | |
| dc.creator | Gamboa,Maria Cecilia | |
| dc.creator | Valenzuela,Pablo D.T | |
| dc.creator | Krauskopf,Erwin | |
| dc.date | 2008-04-01 | |
| dc.date.accessioned | 2019-05-03T12:44:30Z | |
| dc.date.available | 2019-05-03T12:44:30Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85008 | |
| dc.description | Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582008000200008 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.11 n.2 2008 | |
| dc.subject | cellulose | |
| dc.subject | cold-temperature | |
| dc.subject | EST database | |
| dc.subject | forest biotechnology | |
| dc.subject | lignin | |
| dc.title | Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions | |
