| dc.creator | Natarajan,Chandrasekhar | |
| dc.creator | Vijayan,Neetha N | |
| dc.creator | Vaidyan,Linda Koshy | |
| dc.creator | Mathew,Anila | |
| dc.creator | Srinivas,Lekshmy | |
| dc.creator | Banerjee,Moinak | |
| dc.date | 2008-04-01 | |
| dc.date.accessioned | 2019-05-03T12:44:31Z | |
| dc.date.available | 2019-05-03T12:44:31Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85015 | |
| dc.description | Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582008000200015 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.11 n.2 2008 | |
| dc.subject | 100bp ladder | |
| dc.subject | cost effective | |
| dc.subject | DNA size standards | |
| dc.subject | endless usage | |
| dc.title | Universal protocol for generating 100bp size standard for endless usage | |
