| dc.creator | Masani,Mat Yunus Abdul | |
| dc.creator | Parveez,Ghulam Kadir Ahmad | |
| dc.date | 2008-07-01 | |
| dc.date.accessioned | 2019-05-03T12:44:31Z | |
| dc.date.available | 2019-05-03T12:44:31Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85018 | |
| dc.description | The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582008000300003 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.11 n.3 2008 | |
| dc.subject | oleate | |
| dc.subject | transformation vectors | |
| dc.subject | transgenic oil palm | |
| dc.title | Development of transformation vectors for the production of potentially high oleate transgenic oil palm | |
