Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
Author
Acevedo,Cristian
Stach,Matthias H
Amtmann,Anette
Young,Manuel E
Reyes,Juan G
Huebner,Holger
Buchholz,Rainer
Abstract
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.