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dc.creatorFerreira,Susana M. P.
dc.creatorDuarte,Ana P.
dc.creatorQueiroz,João A.
dc.creatorDomingues,Fernanda C.
dc.date2009-07-01
dc.date.accessioned2019-05-03T12:44:37Z
dc.date.available2019-05-03T12:44:37Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300008
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85078
dc.descriptionThe cellulase enzyme production is a key issue in the enzymatic hydrolysis of lignocellulosic materials. Since fungal morphology influences the productivity of fungal fermentations, it is of major importance to well know the fungal behavior during culture for cellulase production. In this work, the influence of medium supplementation, with different buffer systems at two different concentrations and pH conditions, on the morphology of T. reesei Rut C-30 and cellulase production, was investigated. A medium without buffer was used as control. The results suggest that fungal morphology is significantly dependent on the addition of different buffer systems to the nutrient broth. The mycelial morphology shows a clear transition from clumped to pelleted forms in cultures with variation of buffer systems and concentration. The higher filter paper activity was obtained using 100 mM succinate buffer, at pH 4.8, in the medium supplementation, corresponding to a dispersed mycelial morphology.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582009000300008
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.12 n.3 2009
dc.subjectbuffer systems
dc.subjectcellulase
dc.subjectfungal morphology
dc.subjectsubmerged cell growth
dc.subjectTrichoderma reesei Rut C-30
dc.titleInfluence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production


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