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dc.creatorKappell,Guimel M
dc.creatorGrover,James P
dc.creatorChrzanowski,Thomas H
dc.date2009-07-01
dc.date.accessioned2019-05-03T12:44:37Z
dc.date.available2019-05-03T12:44:37Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85080
dc.descriptionThe formation of biofilms on indwelling/implanted medical devices is a common problem. One of the approaches used to prevent biofilm formation on medical devices is to inhibit bacterial attachment by modification of the synthetic polymers used to fabricate the device. In this work, we assessed how micro-scale features (patterns) imprinted onto the surface of silicone elastomer similar to that used for medical applications influenced biofilm formation by Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Patterns were transferred from a multi-patterned oxidized silicon-wafer master-template to silicone elastomer. Features consisted of bars, squares, and circles each extending 0.51 µm above the surface. Feature sizes ranged between 1.78 and 22.25 µm. Distances separating features ranged between 0.26 and 17.35 µm. Bacterial biofilm formation on discs cut from imprinted silicone elastomer was assessed by direct microscopic observation and quantified as the surface area covered by biofilm. Unpatterned silicone elastomer served as a control. Several of the micro-scale patterns imprinted into the silicone elastomer significantly reduced biofilm formation by each bacterium and interrupted biofilm continuity. Although there were differences in detail among strains, bacteria tended to attach in the area between features more than to the surface of the feature itself.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582009000300010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.12 n.3 2009
dc.subjectindwelling medical devices
dc.subjectinfections
dc.subjectsurface attachment
dc.titleMicro-scale surface-patterning influences biofilm formation


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