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dc.creatorQuiroz-Castañeda,Rosa E
dc.creatorBalcázar-López,Edgar
dc.creatorDantán-González,Edgar
dc.creatorMartinez,Alfredo
dc.creatorFolch-Mallol,Jorge
dc.creatorMartínez Anaya,Claudia
dc.date2009-10-01
dc.date.accessioned2019-05-03T12:44:38Z
dc.date.available2019-05-03T12:44:38Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85090
dc.descriptionCellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic activities, with molecular masses ranging from 25 to 90 kDa, from the two species were detected in zymograms.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582009000400005
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.12 n.4 2009
dc.subjectB. adusta
dc.subjectcellulases
dc.subjectP. sanguineus
dc.subjectwheat straw
dc.subjectxylanases
dc.titleCharacterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium


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