In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

dc.creator	Piña-Escutia,José Luis
dc.creator	Vázquez-García,Luis Miguel
dc.creator	Arzate-Fernández,Amaury Martín
dc.date	2010-01-01
dc.date.accessioned	2019-05-03T12:44:40Z
dc.date.available	2019-05-03T12:44:40Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/85104
dc.description	Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
dc.format	text/html
dc.language	en
dc.publisher	Pontificia Universidad Católica de Valparaíso
dc.relation	10.4067/S0717-34582010000100007
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Electronic Journal of Biotechnology v.13 n.1 2010
dc.subject	genetic integrity
dc.subject	micropropagation
dc.subject	oceloxóchitl
dc.title	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.