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dc.creatorRywinska,Anita
dc.creatorRymowicz,Waldemar
dc.creatorMarcinkiewicz,Marta
dc.date2010-07-01
dc.date.accessioned2019-05-03T12:44:44Z
dc.date.available2019-05-03T12:44:44Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85138
dc.descriptionIn the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The volumetric citric acid productivity was markedly higher (1.05 and 0.94 g∙L-1h-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7 strains, respectively) in the cultures containing 200 g∙L-1 of carbon source.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.13 n.4 2010
dc.subjectcitric acid
dc.subjectfed-batch culture
dc.subjectYarrowia lipolytica
dc.subjectraw glycerol
dc.titleValorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast


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