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Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction

dc.creatorYu,Shu Tao
dc.creatorWang,Chuan Tang
dc.creatorYu,Shan Lin
dc.creatorWang,Xiu Zhen
dc.creatorTang,Yue Yi
dc.creatorChen,Dian Xu
dc.creatorZhang,Jian Cheng
dc.date2010-07-01
dc.date.accessioned2019-05-03T12:44:44Z
dc.date.available2019-05-03T12:44:44Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85141
dc.descriptionAn efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.13 n.4 2010
dc.subjectcotyledonary tissue
dc.subjectDNA extraction
dc.subjectgroundnut
dc.subjectPCR
dc.subjectpeanut
dc.titleSimple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction


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