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dc.creatorKantachote,Duangporn
dc.creatorPrachyakij,Pakorn
dc.creatorCharernjiratrakul,Wilawan
dc.creatorOngsakul,Metta
dc.creatorDuangjitcharoen,Yodsawee
dc.creatorChaiyasut,Chaiyavat
dc.creatorNitoda,Teruhiko
dc.creatorKanzaki,Hiroshi
dc.date2010-09-01
dc.date.accessioned2019-05-03T12:44:44Z
dc.date.available2019-05-03T12:44:44Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500002
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85143
dc.descriptionLactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against food borne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10(9) and 10(12) cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 10(12) cells per mouse when compared with the control group.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.13 n.5 2010
dc.subjectfermented plant beverage
dc.subjectLactobacillus plantarum
dc.subjectphenyllactic acid
dc.subjectprobiotics
dc.subjectRhodotorula mucilaginosa
dc.subjectweight control
dc.titleCharacterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages


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