| dc.creator | Fang,Qiangyi | |
| dc.creator | Shen,Bingqian | |
| dc.date | 2010-09-01 | |
| dc.date.accessioned | 2019-05-03T12:44:46Z | |
| dc.date.available | 2019-05-03T12:44:46Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85151 | |
| dc.description | Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency. The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg/mL, a polyethylenimine concentration of 10.5 µg/mL, and an incubation time of 11.8 min. Analysis of variance demonstrated that the experimental values fit well with a quadratic model. The RSM-optimized transient transfection resulted in greater production of human tissue prokallikrein (TproK) than non-RSM-optimized conditions: protein yield was 32.0 mg/L and the maximum viable cell density reached 3.57 x 10(6) cells/mL in a 5 L stirred-tank bioreactor culture. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.13 n.5 2010 | |
| dc.subject | bioreactor | |
| dc.subject | human tissue kallikrein | |
| dc.subject | transient gene expression | |
| dc.title | Optimization of polyethylenimine-mediated transient transfection using response surface methodology design | |
