Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Author
Wang,Chuan Tang
Yu,Shan Lin
Zhang,Shu Wei
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Chen,Dian Xu
Abstract
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.