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dc.creatorTalia,Paola
dc.creatorNishinakamasu,Verónica
dc.creatorHopp,Horacio Esteban
dc.creatorHeinz,Ruth Amelia
dc.creatorPaniego,Norma
dc.date2010-11-01
dc.date.accessioned2019-05-03T12:44:47Z
dc.date.available2019-05-03T12:44:47Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85170
dc.descriptionIn order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDel) and 304 amplified fragment length polymorphisms (AFLP) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.13 n.6 2010
dc.subjectEST-SSR
dc.subjectInDels
dc.subjectlinkage map
dc.subjectSSR
dc.subjectsunflower
dc.titleGenetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map


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