| dc.creator | Saponji,Svetlana | |
| dc.creator | Kneevi-Jugovi,Zorica D | |
| dc.creator | Bezbradica,Dejan I | |
| dc.creator | Zuza,Milena G | |
| dc.creator | Saied,Omar Ali | |
| dc.creator | Boskovi-Vragolovi,Nevenka | |
| dc.creator | Mijin,Dusan Z | |
| dc.date | 2010-11-01 | |
| dc.date.accessioned | 2019-05-03T12:44:47Z | |
| dc.date.available | 2019-05-03T12:44:47Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600012 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85175 | |
| dc.description | Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion (> 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g-1 dry support and in a relatively short time (24 hrs) at 37�C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.13 n.6 2010 | |
| dc.subject | bioreactors | |
| dc.subject | covalent immobilization | |
| dc.subject | ester | |
| dc.subject | non-aqueous system | |
| dc.subject | optimization technique | |
| dc.title | Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study | |
