Biochemical properties of an extracellular &#946;-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate

Rustiguel,Cynthia Barbosa; Oliveira,Arthur Henrique Cavalcanti de; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza

dc.creator	Rustiguel,Cynthia Barbosa
dc.creator	Oliveira,Arthur Henrique Cavalcanti de
dc.creator	Terenzi,Héctor Francisco
dc.creator	Jorge,João Atílio
dc.creator	Guimarães,Luis Henrique Souza
dc.date	2011-03-01
dc.date.accessioned	2019-05-03T12:44:49Z
dc.date.available	2019-05-03T12:44:49Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200002
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/85191
dc.description	The filamentous fungus A. phoenicis produced high levels of ?-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55Ã¯Â¿Â½C and 4.5. The enzyme was stable for more than 1 hr at 50Ã¯Â¿Â½C and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4Ã¯Â¿Â½C by 200 hrs. Dichroism analysis showed the presence of random and ?-sheet structure. A. phoenicis FFase II was activated by Mn2+, Mg2+ and Co2+, and inhibited by Cu2+, Hg2+ and EDTA. The enzyme hydrolyzed sucrose, inulin and raffinose. Kd and Vmax values were 18 mM and 189 U/mg protein using sucrose as substrate.
dc.format	text/html
dc.language	en
dc.publisher	Pontificia Universidad Católica de Valparaíso
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Electronic Journal of Biotechnology v.14 n.2 2011
dc.subject	agroindustrial substrate
dc.subject	Aspergillus
dc.subject	?-D-fructofuranosidase
dc.subject	solid state fermentation
dc.subject	sucrose
dc.title	Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate

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Electronic Journal of Biotechnology
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Biochemical properties of an extracellular &#946;-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate

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Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate