| dc.creator | Kim,Jong-Wan | |
| dc.creator | Yoe,Jeehyun | |
| dc.creator | Lee,Gil Ho | |
| dc.creator | Yoe,Sung Moon | |
| dc.date | 2011-05-01 | |
| dc.date.accessioned | 2019-05-03T12:44:50Z | |
| dc.date.available | 2019-05-03T12:44:50Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300006 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85206 | |
| dc.description | The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95% purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for maximizing production and purification of proteins expressed as inclusion bodies in E. coli. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.3 2011 | |
| dc.subject | antibacterial activity | |
| dc.subject | inclusion body | |
| dc.subject | lysozyme | |
| dc.subject | on-column refolding | |
| dc.subject | recombinant expression | |
| dc.subject | Spodoptera litura | |
| dc.title | Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli | |
