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  • Pontificia Universidad Católica de Valparaíso
  • Electronic Journal of Biotechnology
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A preferable approach to clone hLIF cDNA from the genomic DNA

Author
Cui,Yihong

Zhu,Guangqin

Chen,Qiuju

Wang,Yunfei

Yang,Mingming

Song,Yuxuan

Wang,Jiangang

Cao,Binyun

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012
Abstract
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.
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Discipline
Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
Institutions
Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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