| dc.creator | Domènech,Anna | |
| dc.creator | Pich,Sara | |
| dc.creator | Arís,Anna | |
| dc.creator | Plasencia,Carmen | |
| dc.creator | Bach,Alex | |
| dc.creator | Serrano,Alicia | |
| dc.date | 2011-07-01 | |
| dc.date.accessioned | 2019-05-03T12:44:52Z | |
| dc.date.available | 2019-05-03T12:44:52Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85224 | |
| dc.description | Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17β-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17β-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17β-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17β-estradiol pre-ovulation peak. Conclusions: 17β-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.4 2011 | |
| dc.subject | cattle | |
| dc.subject | estrous | |
| dc.subject | heat | |
| dc.subject | hormone | |
| dc.subject | insemination | |
| dc.title | Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay | |
