| dc.creator | Zhang,Wei-Wei | |
| dc.creator | Yang,Ming-Ming | |
| dc.creator | Li,Heng-xin | |
| dc.creator | Wang,Dun | |
| dc.date | 2011-11-01 | |
| dc.date.accessioned | 2019-05-03T12:44:53Z | |
| dc.date.available | 2019-05-03T12:44:53Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85238 | |
| dc.description | As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 μg/ml VS. 198.89 μg/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 μg/ml VS. 991.59 μg/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.6 2011 | |
| dc.subject | Bacillus subtilis | |
| dc.subject | biosynthesis | |
| dc.subject | integration | |
| dc.subject | Pglv promoter | |
| dc.subject | pimelic acid | |
| dc.subject | single crossover event | |
| dc.title | Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis | |
