| dc.creator | Shan,Shi-hua | |
| dc.creator | Zhang,Ting-ting | |
| dc.creator | Li,Chun-juan | |
| dc.creator | Yang,Chen | |
| dc.creator | Yan,Cai-xia | |
| dc.creator | Wan,Shu-bo | |
| dc.date | 2011-11-01 | |
| dc.date.accessioned | 2019-05-03T12:44:55Z | |
| dc.date.available | 2019-05-03T12:44:55Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600006 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85243 | |
| dc.description | Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.6 2011 | |
| dc.subject | bioinformatics | |
| dc.subject | NBS-LRR | |
| dc.subject | peanut | |
| dc.subject | real-time fluorescence quantitative PCR | |
| dc.title | Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L.) | |
