dc.creator | Díaz,Mauricio | |
dc.creator | Polanco,Victor | |
dc.creator | Ramírez,Ingrid | |
dc.creator | Peña-Cortés,Hugo | |
dc.date | 2012-01-01 | |
dc.date.accessioned | 2019-05-03T12:44:56Z | |
dc.date.available | 2019-05-03T12:44:56Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100010 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85258 | |
dc.description | Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Electronic Journal of Biotechnology v.15 n.1 2012 | |
dc.subject | 12-oxophytodienoate reductase | |
dc.subject | jasmonic acid | |
dc.subject | OPDA | |
dc.subject | Solanum tuberosum | |
dc.subject | stress | |
dc.subject | wounding | |
dc.title | Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum | |