| dc.creator | Ding-ping,Bai | |
| dc.creator | Cheng-jiang,Yu | |
| dc.creator | Yu-lin,Chen | |
| dc.date | 2012-03-01 | |
| dc.date.accessioned | 2019-05-03T12:44:56Z | |
| dc.date.available | 2019-05-03T12:44:56Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85265 | |
| dc.description | Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was firstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the significant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.15 n.2 2012 | |
| dc.subject | AA-NAT gene | |
| dc.subject | polymorphism | |
| dc.subject | reproduction | |
| dc.subject | seasonality | |
| dc.subject | sheep | |
| dc.title | Association between AA-NAT gene polymorphism and reproductive performance in sheep | |
