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dc.creatorNi,Wei
dc.creatorHu,Shengwei
dc.creatorHazi,Wureli
dc.creatorWang,Yuanzhi
dc.creatorQiao,Jun
dc.creatorYan,Ren
dc.creatorChen,Chuangfu
dc.date2012-03-01
dc.date.accessioned2019-05-03T12:44:56Z
dc.date.available2019-05-03T12:44:56Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85268
dc.descriptionBackground: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful biological tool for the regulation of gene expression. For the synthesis of siRNA by vector-based expression systems, several mammalian small nuclear RNA (snRNA) promoters have been cloned and shown different transcriptional efficiencies. Results: In this study, we identified a sheep 7SK snRNA (s7SK) promoter based on the highly conserved polymerase III promoter elements. Promoter activity was measured by promoter-driven shRNA expression to suppress expression of an exogenous reporter gene and endogenous sheep gene. Conclusions: The knock down assay demonstrated that the s7SK induced more stronger inhibition effect than human U6 and H1 promoters. The use of this native sheep 7SK promoter for shRNA expressionis an important component for development of RNAi-based gene therapy and production of transgenic animals in sheep species.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.15 n.2 2012
dc.subject7SK promoter
dc.subjectRNAi
dc.subjectsheep
dc.subjectshRNA
dc.titleSheep 7SK promoter for short hairpin RNA expression


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