Show simple item record

dc.creatorLin,Ling
dc.creatorKan,Xianzhao
dc.creatorYan,Hao
dc.creatorWang,Danni
dc.date2012-05-01
dc.date.accessioned2019-05-03T12:44:56Z
dc.date.available2019-05-03T12:44:56Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85270
dc.descriptionThe gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.15 n.3 2012
dc.subjectBacillus thuringiensis
dc.subjectcellulase
dc.subjectcellulose-degrading
dc.subjectcharacterization
dc.titleCharacterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains


This item appears in the following Collection(s)

Show simple item record