| dc.creator | Ramírez,Ingrid | |
| dc.creator | Dorta,Fernando | |
| dc.creator | Cuadros-Inostroza,Álvaro | |
| dc.creator | Peña-Cortés,Hugo | |
| dc.date | 2012-07-01 | |
| dc.date.accessioned | 2019-05-03T12:44:58Z | |
| dc.date.available | 2019-05-03T12:44:58Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400007 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85284 | |
| dc.description | A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.15 n.4 2012 | |
| dc.subject | antioxidants | |
| dc.subject | callus induction | |
| dc.subject | Fabaceae | |
| dc.subject | gorse | |
| dc.subject | plant regeneration | |
| dc.subject | Ulex europaeus | |
| dc.title | Callus induction and plant regeneration of Ulex europaeus | |
