| dc.creator | Zhang,Yuejin | |
| dc.creator | Qin,Yali | |
| dc.creator | Guo,Lijun | |
| dc.creator | Zhou,Zili | |
| dc.creator | Liang,Zongsuo | |
| dc.creator | Zhang,Chenlu | |
| dc.creator | Guo,Hongbo | |
| dc.date | 2012-09-01 | |
| dc.date.accessioned | 2019-05-03T12:44:59Z | |
| dc.date.available | 2019-05-03T12:44:59Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85296 | |
| dc.description | Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 ± 0.21 ng μl-1. The Trizol method also showed good quality with the yield 469.60 ng μl-1. The SRAP PCR amplified clear and polymorphic bands in all five cDNA samples transcribed from RNA by using primer Me4-Em4. GMP gene fragment (1251 bp) was successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. Conclusion: All these results showed that the total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.15 n.5 2012 | |
| dc.subject | Chuling | |
| dc.subject | medicinal fungus | |
| dc.subject | Polyporus umbellatus | |
| dc.subject | RNA isolation | |
| dc.title | Isolation of high quality RNA from Polyporus umbellatus (Pers.) Fries | |
