| dc.creator | Vergara,Mauricio | |
| dc.creator | Becerra,Silvana | |
| dc.creator | Díaz-Barrera,Alvaro | |
| dc.creator | Berrios,Julio | |
| dc.creator | Altamirano,Claudia | |
| dc.date | 2012-11-01 | |
| dc.date.accessioned | 2019-05-03T12:45:01Z | |
| dc.date.available | 2019-05-03T12:45:01Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85309 | |
| dc.description | We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to the culture at 37ºC. In semi-perfusion culture, the highest rh-tPA specific productivity was obtained at 34ºC. Similarly, whether a decrease in the temperature is accompanied of the replacement of glucose by galactose, the rh-tPA specific productivity improved about 112% over that obtained in semi-perfusion culture carried out at 37ºC with glucose as the carbon source. A semi-perfusion culture strategy was implemented based on the combined effect of the chosen carbon source and low temperatures, which was a useful approach for enhance the specific productivity of the recombinant protein. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.2225/vol15-issue6-fulltext-2 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.15 n.6 2012 | |
| dc.subject | CHO cells | |
| dc.subject | galactose | |
| dc.subject | glutamate | |
| dc.subject | low temperature | |
| dc.subject | perfusion culture | |
| dc.subject | rh-tPA | |
| dc.title | Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA | |
