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dc.creatorElgueta,Sebastián
dc.creatorRubilar,Olga
dc.creatorLima,Nelson
dc.creatorDiez,M. Cristina
dc.date2012-11-01
dc.date.accessioned2019-05-03T12:45:02Z
dc.date.available2019-05-03T12:45:02Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85315
dc.descriptionSix strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.2225/vol15-issue6-fulltext-10
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.15 n.6 2012
dc.subjectcomplex pellets
dc.subjectligninolytic enzymes
dc.subjectRO165 decolourization
dc.subjectwhite-rot fungi
dc.titleSelection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization


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