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dc.creatorBernal,Mauricio
dc.creatorTinoco,Lizeth K
dc.creatorTorres,Luz
dc.creatorMalagón-Romero,Dionisio
dc.creatorMontoya,Dolly
dc.date2013-03-01
dc.date.accessioned2019-05-03T12:45:03Z
dc.date.available2019-05-03T12:45:03Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85328
dc.descriptionBackground: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00). Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production). Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.2225/vol16-issue2-fulltext-4
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.16 n.2 2013
dc.subjectanaerobic fermentation
dc.subjectbiofuel
dc.subjectClostridium spp
dc.subjectglycerol, hydrogen
dc.subjectmathematical model
dc.titleEvaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate


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