| dc.creator | Qi,Zhitao | |
| dc.creator | Shi,Rongwei | |
| dc.creator | Li,Suyang | |
| dc.creator | Zang,Xiayan | |
| dc.creator | Wang,Zisheng | |
| dc.date | 2013-07-01 | |
| dc.date.accessioned | 2019-05-03T12:45:05Z | |
| dc.date.available | 2019-05-03T12:45:05Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400003 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85350 | |
| dc.description | Background: Interleukin-22 (IL-22) plays an important role in the regulation of immune responses. However, little is known about its function or structure in fish. Results: The IL-22 gene was first cloned from So-iny mullet (Liza haematocheila), one of commercially important fish species in China. Then, 3-D structure model of the mullet IL-22 was constructed by comparative modeling method using human IL-22 (1M4R) as template, and a 5 ns molecular dynamics (MD) was studied. The open reading frame (ORF) of mullet IL-22 cDNA was 555 bp, encoding 184 amino acids. The mullet IL-22 shared higher identities with the other fish IL-22 homologs and possessed a conserved IL-10 signature motif at its C-terminal. The mullet IL-22 model possessed six conserved helix structure. PROCHECK, SAVES and Molprobity server analysis confirmed that this model threaded well with human IL-22. Strikingly, analysis with CastP, cons-PPISP server suggested that the cysteines in mullet IL-22 might not be involved in the forming of disulfide bond for structural stabilization, but related to protein-protein interactions. Conclusions: The structure of IL-22 in So-iny mullet (Liza haematocheila) was constructed using comparative modeling method which provide more information for studying the function of fish IL-22. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.2225/vol16-issue4-fulltext-3 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.16 n.4 2013 | |
| dc.subject | comparative modeling | |
| dc.subject | Interleukin-22 | |
| dc.subject | Liza haematocheila | |
| dc.subject | molecular dynamics | |
| dc.title | 3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila | |
