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dc.creatorZhong,Juan
dc.creatorZhang,Xiaoyong
dc.creatorRen,Yanli
dc.creatorYang,Jie
dc.creatorTan,Hong
dc.creatorZhou,Jinyan
dc.date2014-05-01
dc.date.accessioned2019-05-03T12:45:11Z
dc.date.available2019-05-03T12:45:11Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85414
dc.descriptionBackground Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor. Results The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation as compared to the production of batch fermentation. Conclusion The results of this work improve the present production of JAA and may be adopted for other objective products' production.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.1016/j.ejbt.2014.04.010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.17 n.3 2014
dc.subjectBacillus subtilis ZK8
dc.subjectIturin A
dc.subjectResponse surface methodology
dc.titleOptimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design


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