| dc.creator | Ding,Fang | |
| dc.creator | Yuan,Xin | |
| dc.creator | Li,Qingqing | |
| dc.creator | Sun,Wenqiang | |
| dc.creator | Gan,Chao | |
| dc.creator | He,Hua | |
| dc.creator | Song,Chenling | |
| dc.creator | Wang,Jiwen | |
| dc.date | 2014-11-01 | |
| dc.date.accessioned | 2019-05-03T12:45:13Z | |
| dc.date.available | 2019-05-03T12:45:13Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600001 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85433 | |
| dc.description | Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 µM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05). Conclusion We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.1016/j.ejbt.2014.08.001 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.17 n.6 2014 | |
| dc.subject | Expression pattern | |
| dc.subject | FAS | |
| dc.subject | Oleic acid | |
| dc.subject | Peking duck | |
| dc.title | Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation | |
