dc.creator | Yang,Weiping | |
dc.creator | Meng,Fanxu | |
dc.creator | Peng,Jiayin | |
dc.creator | Han,Peng | |
dc.creator | Fang,Fang | |
dc.creator | Ma,Li | |
dc.creator | Cao,Binyun | |
dc.date | 2014-11-01 | |
dc.date.accessioned | 2019-05-03T12:45:13Z | |
dc.date.available | 2019-05-03T12:45:13Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85434 | |
dc.description | Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24-48 h. Conclusions The isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | |
dc.relation | 10.1016/j.ejbt.2014.08.002 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Electronic Journal of Biotechnology v.17 n.6 2014 | |
dc.subject | Carboxymethyl cellulose | |
dc.subject | Cellulolytic bacterium | |
dc.subject | Fermentation conditions | |
dc.subject | Screening and identification | |
dc.subject | Tibetan pig | |
dc.title | Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production | |