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dc.creatorYang,Weiping
dc.creatorMeng,Fanxu
dc.creatorPeng,Jiayin
dc.creatorHan,Peng
dc.creatorFang,Fang
dc.creatorMa,Li
dc.creatorCao,Binyun
dc.date2014-11-01
dc.date.accessioned2019-05-03T12:45:13Z
dc.date.available2019-05-03T12:45:13Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85434
dc.descriptionBackground The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24-48 h. Conclusions The isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.1016/j.ejbt.2014.08.002
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.17 n.6 2014
dc.subjectCarboxymethyl cellulose
dc.subjectCellulolytic bacterium
dc.subjectFermentation conditions
dc.subjectScreening and identification
dc.subjectTibetan pig
dc.titleIsolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production


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