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dc.creatorXiao,Anfeng
dc.creatorHuang,Yufeng
dc.creatorNi,Hui
dc.creatorCai,Huinong
dc.creatorYang,Qiuming
dc.date2015-05-01
dc.date.accessioned2019-05-03T12:45:16Z
dc.date.available2019-05-03T12:45:16Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85469
dc.descriptionBackground A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R²) of 0.9340, which implied an adequate credibility of the model.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.1016/j.ejbt.2015.02.001
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.18 n.3 2015
dc.subjectAspergillus tubingensis
dc.subjectProcess optimization
dc.subjectResponse surface methodology
dc.subjectTea stalks
dc.subjectTannase fermentation
dc.titleStatistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks


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