dc.creator | Xiao,Anfeng | |
dc.creator | Huang,Yufeng | |
dc.creator | Ni,Hui | |
dc.creator | Cai,Huinong | |
dc.creator | Yang,Qiuming | |
dc.date | 2015-05-01 | |
dc.date.accessioned | 2019-05-03T12:45:16Z | |
dc.date.available | 2019-05-03T12:45:16Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85469 | |
dc.description | Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R²) of 0.9340, which implied an adequate credibility of the model. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | |
dc.relation | 10.1016/j.ejbt.2015.02.001 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Electronic Journal of Biotechnology v.18 n.3 2015 | |
dc.subject | Aspergillus tubingensis | |
dc.subject | Process optimization | |
dc.subject | Response surface methodology | |
dc.subject | Tea stalks | |
dc.subject | Tannase fermentation | |
dc.title | Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks | |