| dc.creator | Makhdum Munawar,Khalil Munawar | |
| dc.creator | Simarani,Khanom | |
| dc.creator | Annuar,Mohamad Suffian Mohamad | |
| dc.date | 2016-01-01 | |
| dc.date.accessioned | 2019-05-03T12:45:22Z | |
| dc.date.available | 2019-05-03T12:45:22Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100008 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85531 | |
| dc.description | Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling. Results: The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7,28°C, aeration surface-to-volume ratio of 0.4 x 10(6) m-1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking-Piret kinetic model. Conclusion: The findings of this study provided add to the literature on key variables in for achieving good microbial growth and mcl-PHA production in shake flasks culture. In addition, suitable kinetic model to describe cultivation in this system was also presented. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.1016/j.ejbt.2015.07.005 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.19 n.1 2016 | |
| dc.subject | Biopolymer | |
| dc.subject | Fermentation | |
| dc.subject | Medium-chain-length PHA | |
| dc.subject | Palm kernel oil | |
| dc.subject | Pseudomonas putida | |
| dc.title | Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks | |
