| dc.creator | Jin,Yuan-yuan | |
| dc.creator | Li,Ya-dong | |
| dc.creator | Sun,Wan | |
| dc.creator | Fan,Shuai | |
| dc.creator | Feng,Xiao-zhou | |
| dc.creator | Wang,Kang-you | |
| dc.creator | He,Wei-qing | |
| dc.creator | Yang,Zhao-yong | |
| dc.date | 2016-05-01 | |
| dc.date.accessioned | 2019-05-03T12:45:24Z | |
| dc.date.available | 2019-05-03T12:45:24Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300004 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85542 | |
| dc.description | Background: D-Hydroxyphenylglycine is considered to be an important chiral molecular building-block of antibiotic reagents such as pesticides, and β-lactam antibiotics. The process of its production is catalyzed by D-hydantoinase and D-carbamoylase in a two-step enzyme reaction. How to enhance the catalytic potential of the two enzymes is valuable for industrial application. In this investigation, an Escherichia coli strain genetically engineered with D-hydantoinase was immobilized by calcium alginate with certain adjuncts to evaluate the optimal condition for the biosynthesis of D-carbamoyl-p-hydroxyphenylglycine (D-CpHPG), the compound further be converted to D-hydroxyphenylglycine (D-HPG) by carbamoylase. Results: The optimal medium to produce D-CpHPG by whole-cell immobilization was a modified Luria-Bertani (LB) added with 3.0% (W/V) alginate, 1.5% (W/V) diatomite, 0.05% (W/V) CaCl2 and 1.00 mM MnCl2.The optimized diameter of immobilized beads for the whole-cell biosynthesis here was 2.60 mm. The maximized production rates of D-CpHPG were up to 76%, and the immobilized beads could be reused for 12 batches. Conclusions: This investigation not only provides an effective procedure for biological production of D-CpHPG, but gives an insight into the whole-cell immobilization technology. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.1016/j.ejbt.2016.01.004 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.19 n.3 2016 | |
| dc.subject | Calcium alginate | |
| dc.subject | d-Carbamoyl-p-hydroxyphenylglycine | |
| dc.subject | d-Hydantoinase | |
| dc.subject | d-Hydroxyphenylglycine | |
| dc.subject | Immobilization | |
| dc.subject | Whole cell | |
| dc.title | The whole-cell immobilization of D-hydantoinase-engineered Escherichia coli for D-CpHPG biosynthesis | |
