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dc.creatorParra,Bárbara
dc.creatorRobeson,James
dc.date2016-05-01
dc.date.accessioned2019-05-03T12:45:24Z
dc.date.available2019-05-03T12:45:24Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300011
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85549
dc.descriptionBackground: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 wastewater samples, collected at different sites in the V Region in Chile, we selected three bacteriophages (FC, FP, and FQ) capable of productively infecting Salmonella enterica serovar Choleraesuis. By transmission electron microscopy (TEM) observation, the bacteriophages were found to belong to the order Caudoviridae. Molecular analyses indicated that FC, FP, and FQ contained double-stranded DNA genomes, of sizes similar to bacteriophage P22, and distinct recognition sites for the restriction endonucleases HaeIII and HindIII. Assays of host range revealed that the bacteriophages were polyvalent and thus capable of infecting different strains of Escherichia coli and other serovars of Salmonella. Conclusion: We have isolated new bacteriophages of the serovar Choleraesuis with various potential applications in relation to this pathogenic bacterium.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.1016/j.ejbt.2016.01.008
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.19 n.3 2016
dc.subjectBacteriophage
dc.subjectPolyvalent
dc.subjectSalmonella enterica serovar Choleraesuis
dc.titleSelection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis


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