| dc.creator | Ghaffar Shahriari,Amir | |
| dc.creator | Bagheri,Abdolreza | |
| dc.creator | Bassami,Mohammad Reza | |
| dc.creator | Malekzadeh-Shafaroudi,Saeid | |
| dc.creator | Afsharifar,Alireza | |
| dc.creator | Niazi,Ali | |
| dc.date | 2016-07-01 | |
| dc.date.accessioned | 2019-05-03T12:45:25Z | |
| dc.date.available | 2019-05-03T12:45:25Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85557 | |
| dc.description | Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Integration of the foreign gene in plant genome was confirmed by PCR. Expression of foreign gene was analyzed at transcription level by RT-PCR and at translation level by means of dot blotting and ELISA. All analyses confirmed the expression of recombinant protein. Conclusion: Developments in genetic engineering have led to plant-based systems for recombinant vaccine production. In this research, tobacco plant was used to express F and HN epitopes of NDV. Our results indicate that for the production of recombinant vaccine, it is a novel strategy to use concatenated epitopes without their genetic fusion onto larger scaffold structure such as viral coat protein. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.1016/j.ejbt.2016.05.003 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.19 n.4 2016 | |
| dc.subject | Agrobacterium tumefaciens | |
| dc.subject | ELISA | |
| dc.subject | Recombinant vaccine | |
| dc.subject | Transgenic plants | |
| dc.title | Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco | |
