| dc.creator | Xu,Ji-Fei | |
| dc.creator | Mi,Yuan-Ting | |
| dc.creator | Ren,Nan-Qi | |
| dc.date | 2016-09-01 | |
| dc.date.accessioned | 2019-05-03T12:45:26Z | |
| dc.date.available | 2019-05-03T12:45:26Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85566 | |
| dc.description | The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH. At buffer concentrations 100-250 mM, the maximum hydrogen production was barely suppressed, and the lag phase extended past 7 h. Therefore, although acetate inhibits hydrogen production, using acetate as a buffer (like phosphate) effectively prevented pH drops and increased substrate consumption, enhancing hydrogen production. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.1016/j.ejbt.2016.07.002 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.19 n.5 2016 | |
| dc.subject | Acetate | |
| dc.subject | Biohydrogen | |
| dc.subject | Buffering action | |
| dc.subject | Ethanoligenens harbinense phosphate | |
| dc.title | Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49 | |
