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dc.creatorTumkur Ramasetty,Bharathi
dc.creatorNaik Bajpe,Shrisha
dc.creatorKigga Kadappa,Sampath Kumara
dc.creatorKumar Saini,Ramesh
dc.creatorNittur Basavaraju,Shashibhushan
dc.creatorKukkundoor Ramachandra,Kini
dc.creatorHarishchandra Sripathy,Prakash
dc.date2016-11-01
dc.date.accessioned2019-05-03T12:45:27Z
dc.date.available2019-05-03T12:45:27Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600001
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85575
dc.descriptionBackground: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.1016/j.ejbt.2016.09.001
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.19 n.6 2016
dc.subjectatpF-atpH
dc.subjectMemecylon species
dc.subjectndh
dc.subjectpsbA-trnH
dc.subjectrpoC1
dc.subject5s
dc.subjectPCR based techniques
dc.subjectMolecular phylogenetic analysis
dc.subjectMolecular phylogenesis
dc.subjectDNA markers
dc.titleIdentification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-Based DNA barcoding tools


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