Aloe vera has been used worldwide both for pharmaceutical, food, and cosmetic industries due to the plethora of biological activities of some of its metabolites. A study case is reported focusing on the development of a cloning protocol of A. vera to provide propagation material with superior quality to the private sector in southern Brazil, i.e., A. vera juice industry. Such biotechnological approach afforded ca. 4,300 plantlets from 20 explants, over a 6-month period, overcoming the drawback of the lack of propagation material. Typically, the results have led to the increase of the cultured area and juice production of that species coming true the goal of the partnership between the public and private sectors herein involved. The transference of the resulting technology was successfully performed to the company and a patent covering the biotechnological process has been recently requested to official organisms on behalf of the partners.El Aloe vera ha sido empleado mundialmente en las industrias farmacéutica, alimentaria y cosmética, debido a la variedad de actividades biológicas de sus metabolitos. Un estudio de caso es reportado, enfocado en el desarrollo de un protocolo de clonamiento de A. vera para proveer propagación de material de calidad superior al sector privado en el sur de Brasil, por ejemplo, la industria de jugo de A. Vera. Ya que las plantas obtenidas mediante métodos biotecnológicos ascienden a 4300 plántulas desde 20 explantes, en un período de 6 meses, se hace un problema la inminente falta de material. Típicamente los resultados han conducido al incremento de del área cultivada y de la producción de jugo de las especies con verdaderos objetivos de asociatividad entre los sectores privados y públicos. La transferencia de la tecnología resultante fue exitosamente desempeñada hacia la compañía y una patente que protege el proceso biotecnológico ha sido recientemente solicitada a los organismos de patentamiento.Aloe vera has been used worldwide both for pharmaceutical, food, and cosmetic industries due to the plethora of biological activities of some of its metabolites. A study case is reported focusing on the development of a cloning protocol of A. vera to provide propagation material with superior quality to the private sector in southern Brazil, i.e., A. vera juice industry. Such biotechnological approach afforded ca. 4,300 plantlets from 20 explants, over a 6-month period, overcoming the drawback of the lack of propagation material. Typically, the results have led to the increase of the cultured area and juice production of that species coming true the goal of the partnership between the public and private sectors herein involved. The transference of the resulting technology was successfully performed to the company and a patent covering the biotechnological process has been recently requested to official organisms on behalf of the partners.